The Amazing Human Microbiome Thursday, March 28 2013, 11am Read more about The Amazing Human Microbiome
Applied Microbiology and Biotechnology Biotechnology, biofuels, biocatalysts, fermentation, food microbiology Read more about Applied Microbiology and Biotechnology
Microbial Ecology Interactions of microorganisms with the environment, and their roles in natural communities Read more about Microbial Ecology
Microbe-Host Interactions Roles of microbes in disease, beneficial symbiosis, pathogenesis, parasitology, and immunology Read more about Microbe-Host Interactions
Bioinformatics and -omics/Computational Biology Elucidating biological phenomena by analyzing nucleic acid and protein sequences from microbes and microbial communities Read more about Bioinformatics and -omics/Computational Biology
Microbial Physiology Diversity of metabolic and energy-generating pathways. Read more about Microbial Physiology
Whitman Research Interests: Our research examines the physiology, systematics and ecology of environmentally important microorganisms, but especially the methane-producing archaea and the marine alpha proteobacteria. Of special interest is to understand the complete organism, from the biochemical mechanisms it uses to accomplish its day-to-day tasks of living to its interactions with other organisms and its environment in general. Every organism has its own story. The methanogens are strictly anaerobic autotrophs which obtain energy from the synthesis of methane gas. They catalyze the terminal step in the anaerobic decomposition of organic matter, and about 1.6 % of the CO2 fixed worldwide is released to the atmosphere by the activity of these bacteria. As autotrophs they are also unusual because they are commonly found closely associated with heterotrophic bacteria and protists. Our studies of the methanogens have focused on Methanococcus, which is common in estuarine environments. Currently, we are using genetic methods to elucidate novel biosynthetic pathways and mechanisms of H2 metabolism. The roseobacters play important roles in the transformation of carbon and sulfur compounds in the ocean and estuarine environments. Currently, we are studying the metabolism of dimethylsulfonium propionate (DMSP), a common osmolyte and major source of atmospheric sulfur, by Siliicibacter pomeroyii. Studies of this model organism provide insights into the mechanisms of DMSP degradation in the natural environments. Read more about Whitman
Summers Research Interests: We have long studied bacterial plasmid-encoded resistance to inorganic and organic mercury compounds (the mer locus) as a model for (a) gene regulation by toxic metals, (b) microbial detoxification of environmental hazards, and (c) the influence of toxic metals on the commensal microbiota of vertebrates. Our present work on mer focuses on structure-function and dynamic studies of the two major types of MerR protein and on the unique interactions of the classical Tn21 MerR regulator with RNA polymerase during repression and activation and with MerD during shut-down of mer operon expression. In this work we collaborate with the groups of Jeremy Smith and Liyuan Liang at the Oak Ridge National Laboratory. We also have a growing interest in the molecular basis of mercury intoxication. With collaborators Sue Miller at UCSF and Mary Lipton at the Pacific Northwest National Laboratory we have devised a computational filter based on the seven stable isotopes of Hg to identify, at the individual peptide scale using LC-MS/MS proteomics, those proteins most vulnerable to forming adducts with organo-mercurials. We are now beginning a new project to similarly define the inorganic mercury "exposome" and to use this approach in collaboration with Judy Wall at Missouri for high-throughput discovery of proteins involved in mercury methylation by sulfate reducing bacteria. We have also worked in the area of lateral gene transfer in prokaryotes with special emphasis on the dissemination by plasmids and transposons of genes for resistance to toxic metals and antibiotics. We are now wrapping up two major sequencing projects on large, mobile plasmids of meticillin-resistant Staphylococcus aureus (MRSA) and of several genera of marine bacteria, agricultural pathogens, and bacteria important in biofuel fermentation. Read more about Summers
Stabb The Stabb Lab: Researchers in Dr. Eric Stabb's lab study the light-organ symbiosis between the bioluminescent bacterium Vibrio fischeri and the squid Euprymna scolopes, as a model for natural bacteria-animal interactions. E. scolopes hatchlings lack V. fischeri, which they must obtain from their surroundings. After infection, the squid carry V. fischeri, and only this bacterium, in epithelium-lined crypts of a specialized light-emitting organ. Several features make this symbiosis uniquely tractable. Notably, this natural infection can be reconstituted in the lab, so we are able to observe the bacteria and their gene expression in an ecologically relevant context inside its host. One focus of the lab is how V. fischeri cells communicate using pheromone signals. Such bacterial signaling is often termed "quorum sensing" and is typically depicted as regulation in response to high cell density or "quorum". However, it is now clear that the pheromones are not simply census-taking molecules and can perform more complicated social roles. The pheromones in V. fischeri control the lux genes responsible for bioluminescence, and the pheromones are controlled by themselves in positive-feedback loops and by environmentally responsive regulators, including ArcA/ArcB, Crp, Fur, and others. This raises the intriguing possibility that in addition to reflecting cell density, bacterial pheromones may coordinate behaviors, such as the group decision to bioluminesce, in response to local environmental cues. Interestingly, bioluminescence is induced upon entering the symbiosis, and dark (lux) mutants are attenuated in colonizing the E. scolopes light organ; however, the symbiotic role of bioluminescence remains unclear. Studying pheromone-mediated regulation may help shed light on the purpose of bioluminescence. We have embarked on a collaborative project to model and understand how V. fischeri uses multiple pheromones to underpin its cell-cell communication. A second major focus of the lab is aimed at understanding the interspecies signaling by which the squid host recognizes and responds to V. fischeri. Peptidoglycan and LPS can trigger changes in host development that parallel those seen during normal symbiotic infection. We are interested in how and why V. fischeri releases peptidoglycan monomer, which acts as a morphogen on the host. Currently, a project in the lab is using V. fischeri as a model to understand how new peptidoglycan structures can evolve in bacteria. Many projects in the lab are underpinned by genetic approaches, and we are often developing genetic and genomic tools for V. fischeri. For example, characterizing the small V. fischeri plasmid pES213 led to an array of shuttle vectors that is still expanding. We have also pioneered improved methods for mutagenesis with mini-Tn5 in V. fischeri. Finally, everyone in the Stabb lab contributes to the teaching mission at the University of Georgia, and we are involved in outreach efforts to K-12 students, spreading the word of the power of microbes and the wonders of bioluminescence. Three Stabb labbers [Noreen Lyell, Richard M. (Mark) Jones, and Julie Stoudenmire] have earned the prestigious UGA Excellence in Teaching Award, which is given to only five teaching assistants across the entire university each year. Read more about Stabb
Neidle Research Interests: Research in the Neidle group centers on gene expression, metabolism, and chromosomal rearrangements in a soil bacterium, Acinetobacter baylyi ADP1. A novel method to study and generate chromosomal gene amplification is used for metabolic engineering and experimental evolution. This research has medical implications because gene amplification is associated with drug resistance, bacterial virulence, cancer, and many human diseases, as published in review articles that we wrote in 2013 and 2007. Additionally, there are important biotechnology and environmental applications of this work. Gene amplification in strain ADP1 was first discovered during studies of the degradation of aromatic compounds, as we reported in 2003 and 2004. We are now investigating how these degradative pathways can be exploited to convert lignin-derived aromatic compounds into biofuels and other types of desirable compounds. Pathways for aromatic compound catabolism also hold great promise for reducing pollution (bioremediation). We described the features of ADP1 that make it an ideal experimental bacterium for fundamental and applied research in 2011. Complementary investigations of gene expression and metabolic regulation are ongoing. Two LysR-type transcriptional regulators, BenM and CatM, control the expression of many different genes and operons needed for aromatic compound catabolism. Together with Dr. Cory Momany and his research team, we are investigating the molecular details of the function and structure of LysR-type proteins, a large family of homologs that represent the most common type of bacterial transcriptional regulator. Selected Publications: Elliott, K.T., Cuff, L.E., and Neidle, E.L. (2013) Copy number change: evolving views on gene amplification. Future Microbiology 8:887-899. Alanazi, A.M., Neidle, E.L., and Momany, C. (2013) The DNA-binding domain of BenM reveals the structural basis for the recognition of a T-N11-A sequence motif by LysR-type transcriptional regulators. Acta Crystallographica Section D-Biological Crystallography 69:1995-2007. Seaton, S.C., Elliott, K.T., Cuff, L.E., Laniohan, N.S., Patel, P.R., and Neidle, E.L. (2012) Genome-wide selection for increased copy number in Acinetobacter baylyi ADP1: locus and context-dependent variation in gene amplification. Molecular Microbiology 83:520-535. Momany, C., and Neidle, E.L. (2012) Defying stereotypes: the elusive search for a universal model of LysR-type regulation. Molecular Microbiology 83:453-456. Elliott, K.T., and Neidle, E.L. (2011) Acinetobacter baylyi ADP1: Transforming the Choice of Model Organism. IUBMB Life 63: 1075-1080. Craven, S.H., Ezezika, O.C., Haddad, S., Hall, R.A., Momany, C., and Neidle, E.L. (2009) Inducer responses of BenM, a LysR-type transcriptional regulator from Acinetobacter baylyi ADP1. Molecular Microbiology 72: 881-894. Read more about Neidle